Description
It is known that amyloid-beta, which is the major constituent of senile plaques, is produced from amyloid-beta Precursor Protein (APP), which exists in three main isoforms, namely APP695, APP751, and APP770, by the beta-secretase (BACE1) and subsequently the gamma-secretase.
The production of soluble APP (sAPP) by beta-secretase cleavage is the first step in APP processing. Furthermore, it is has been shown that a specific APP gene mutation exists in individuals who suffer early-onset familial Alzheimer’s disease, which is called Swedish mutation. This gene mutation is a double mutation at positions -1 to -2 from the β-secretase cleavage site (Lysine670→Asparagine and Methionine671→Leucine). This kit can measure the total amount human soluble APP fragments in biological samples.